hes2 cell line  (WiCell Research Institute Inc)

Journal: bioRxiv

Article Title: Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties in vitro and in vivo

doi: 10.1101/2025.09.04.674322

Figure Lengend Snippet: A. Representative flow cytometric analysis and corresponding summary of CD235a and PDGFRα expression in day 4 mesoderm following induction with the specified concentrations of BMP4 (B) and Activin A (A) [ng/ml] in the HES2 cell line (n = 14). B. Representative flow cytometric analysis and corresponding summary of CD235a and PDGFRα expression in day 4 mesoderm following induction with the specified concentrations of BMP4 (B) and Activin A (A) [ng/ml] in the ESI017 ASAP1 cell line (n = 15-16). C. Representative flow cytometric analysis and corresponding summary of cTNT and MLC2V expression in day 20 populations generated with the indicated amounts of BMP4 (B) and Activin A (A) [ng/ml] during mesoderm induction (days 1-3) in the HES2 cell line (n = 14). D. Representative flow cytometric analysis and corresponding summary of cTNT and MLC2V expression in day 20 populations generated with the indicated amounts of BMP4 (B) and Activin A (A) [ng/ml] during mesoderm induction (days 1-3) in the ESI017 ASAP1 cell line (n = 15-16). E. Representative flow cytometric analysis for the expression of cTNT in day 20 cultures treated with either 12 μM of CHIR or 200 ng/mL of BMP2 at the indicated timepoints in the HES2 cell line. F. Bar graph summarizing the proportion of cTNT + cells shown in F (n = 14). G. Representative flow cytometric analysis for the expression of cTNT in day 20 cultures treated with either 12 μM of CHIR or 200 ng/mL of BMP2 at the indicated timepoints in the ESI-017 ASAP1 cell line. H. Bar graph summarizing the the proportion of cTNT + cells shown in G (n = 9). I. Heatmap showing differences in RT-qPCR gene expression levels across HES3, HES2 and ASAP1 AVNLPCs treated with either 12 μM of CHIR (CHIR AVNLPCs) or 200 ng/mL of BMP2 (BMP2 AVNLPCs). VLCMs differentiated from each cell line were included as an expression reference. All populations were analyzed at day 20 of differentiation. Values represent log2 of expression levels relative to the housekeeping gene TBP. J. Immunofluorescent staining of MSX2 in BMP2 AVNLPCs and VLCMs from the HES2 and ESI-017 ASAP1 cell lines. Cells were counterstained with cTNT to visualize all cardiomyocytes and DAPI to visualize all cells. Scale bars, 100 μm. K. Quantification of the percentage of MSX2 + cells determined by immunofluorescence staining in BMP2 AVNLPCs and VLCMs from the HES2 and ESI-017 ASAP1 cell lines (n = 4-5 images per monolayer, taken from 3 independent differentiations). Error bars represent SEM. Statistical tests: One-way ANOVA, Bonferroni’s post hoc test (F, H); unpaired two-sided t-test (A-D, K): *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The HES2 cell line (karyotype: 46, XX) was purchased from WiCell.

Techniques: Expressing, Generated, Quantitative RT-PCR, Gene Expression, Staining, Immunofluorescence

Journal: bioRxiv

Article Title: Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties in vitro and in vivo

doi: 10.1101/2025.09.04.674322

Figure Lengend Snippet: A. Schematic of the BMP2 AVNLPCs differentiation protocol in the HES2 cell line and subsequent processing on the 10x Genomics Chromium platform for scRNA-seq. B. UMAP of day 25 hPSC-derived AVNLPCs showing 5 cell clusters (left). UMAP depicting TNNT2 + clusters (right). C. Spearman correlation between selected cell clusters from the fetal AVN and hPSC-derived AVNLPC dataset. D. GO enrichment analysis of biological processes associated with the positive differentially expessed genes in the hPSC-derived AVNLPC cardiomyocyte cluster. E. UMAP of subclustered TNNT2 + cardiomyocytes showing 4 subclusters. F. UMAPs of the subclustered cardiomyocytes depicting the expression of the indicated genes. G. UMAPs showing signature score distribution for the DEGs of the indicated fetal AVN cell types. H. Spearman correlation between selected cell clusters from the fetal AVN and hPSC-derived AVNLPC datasets, p < 0.05 for all correlations. I. UMAPs showing signature score distribution for the DEGs of indicated fetal heart cell types from the Farah et al. spatial MERFISH dataset. J. UMAPs showing signature score distribution for the DEGs of indicated adult heart cell types from the Kanemaru et al. dataset. K. Heatmap showing the top 5 differentially expressed genes of the AVNLPC cardiomyocyte subtypes.

Article Snippet: The HES2 cell line (karyotype: 46, XX) was purchased from WiCell.

Techniques: Derivative Assay, Expressing